human aortic fibroblasts  (PromoCell)

Journal: Cell Transplantation

Article Title: Inhibiting Matrix Metalloproteinase by Cell-Based Timp-3 Gene Transfer Effectively Treats Acute and Chronic Ischemic Cardiomyopathy

doi: 10.3727/096368911x601000

Figure Lengend Snippet: Figure 6. Histology: hematoxylin and eosin stain. After 2 weeks in culture, postaddition of just the HuSMC cell layer or addition of both the human-derived smooth muscle cells (HuSMC) and human-derived, aortic, adventitial fibroblasts (HAoAFB) layers, the tissue-engineered vascular grafts (TEVBs) were fixed in 10% formalin solution overnight, embedded within paraffin, and sectioned into 4-µm sections. The top two figures show an H&E stain following 2 weeks in culture with the scaffold (acellularized aorta, left, and chitosan tubule, right) and the first cell layer, HuSMC. The bottom two figures also show an H&E stain, 2 weeks after the addition of the second layer of cells, HAoAFB to the circumference of HuSMC layer.

Article Snippet: Primary human aortic adventitial ting voltage of 3 kV.fibroblasts (HAoAFb) were purchased from PromoCell (Cat.# C-12380, Lot# 6090101.22; Heidelberg, Ger- Fabrication of Vascular Graft many), isolated from a 47-year-old Caucasian male Plates were prepared as described above.

Techniques: H&E Stain, Derivative Assay, Staining